However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 130009WThe SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Outer wells aligned for proper viewing with FLIPR Ideal. 3% in white 96- and 384-well microplates Time. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 3. 2. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. 2. This is equivalent to having 511 built-in filters, 384-well: 0. 0 out of 5 $ 14,499. Molecular Devices, LLC. Plate readers, also known as. #4TI-0655) Microplates. 5in (46. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. ". High base plate design is compatible with automation and for bar coding. Gemini XPS Reader scans. in every plate. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. the use of a reader calibrated with primary NIST and NMI standards. Plate Formats: 6- to 384-well plates. 2. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. White plates reflect the light, enabling even low levels of Luminescence to be detected. 384-well TC plate, Poly-d-Lysine, Flat Clear. The. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 012 fmol 0. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells. research needs. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. Instructions for adding new plates: CSI/CP2: Add plate. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. Figure 1. Versa Max. for ELISAs and most cell-based assays. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. x. 20 attomol ATP per well. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Washers configured for both 96- and 384-well plates. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. It has a. We also prefer that fluorescence signals in one well don’t bleed into the other, as this means less background and more precise data. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. Rating: 3. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. M5. Not for use in diagnostic procedures. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. +1 800. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. XML file. Get Quote . 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. X. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 96-, 384-, and 1536. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. 5 mP 1536–2. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. 3860 N First Street. Overview of 384 Well SBS. Experience the identifiably different. We would like to show you a description here but the site won’t allow us. MultiWash+ Microplate Washer. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Reader Documentation Version No. VMax . tools to expand the boundaries of their research. 2. 2, prime the machine with CellTiter-Glo ® 2. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. $542. Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. Includes PC and software. Molecular Devices: n/a:. Another consideration, says Amouretti, is plate format. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. #ABS Plus). UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 20 attomol ATP per well. Microplates, cuvettes (via adapter) Detectors. Method 1. A = 2 - log 10 (%T). #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Figure 3. EMax. Supplier: Molecular Devices. Detailed thermal cycling conditions are listed in Table 2. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 0. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. A control well was set up to verify. Artel MVS Bundle DM Std/800TSNB Reader Details. Features. 2. Filter. 6-384 (96- and 384-well with pipetting) 96-well microplates. Fluorescence Microplate Reader Comparison Chart. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 384-Deep Square Well Plates. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. 6-384 (96- and 384-well with pipetting) 96-well microplates. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Cell viability는 세포막 Integrity 또는. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Turn on Plate Reader and wait for it to complete its start-up routine. There is no product in the shopping cart, buy it!. x. In addition, SoftMax Pro SoftwareAfter incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 0200-6262. 312 gain calibration failed. If you have an xml plate object, then. For nearly 30 years, Molecular Devices has provided scientists with . Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Three low volume 24-well bottom slides. Molecular Devices Spectramax. MultiWash+ Microplate Washer. Service Contracts, Preventative Maintenance and. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. After reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. 6% for the on-board optics (monochromators). Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 5577. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. 0 . Each kit is sufficient. Summary of Contents for Molecular Devices SpectraMax M2. 1. X. Important things to consider when purchasing a multimode microplate reader. 0nm increments. QC tools and IQOQ services. 12, 24, 96 & 384-well SBS microplates; Range of 96, 384-well SBS microplates . changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. 8 mm distance between well bottoms to plate bottom. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Microplates for applications that require high sample recovery rates. In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. SpectraMax ID5 Plate Reader (Molecular Devices). In simplest terms, a plate reader measures and. The former have been the traditional domain of plate readers and great strides have been. Source plate capacity; 1 x plates. Catalog number: AB1384W. 384-Well Fully Skirted Plates, Barcoded, including Armadillo 384-Well PCR plates. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Next-generation luminescence plate reader. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The FilterMax F3 can read 96-well and 384-well plates. M2. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. flat-bottom: 50-60 µl. 6-well tissue-culture treated (TC) plate, sterile. UV absorbance detection. When the user applies a set temperature of 37°C, the top is heated to 37. DynaPro ™ Plate Reader III. FlexStation 3 Multi Mode Microplate Reader. Ensure data accuracy of your reader. 1. 48 W x 12. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. #PLUS 384) Methods. 96- and 384-well . Formats available are strip washers, full plate washers and combination washer-dispensers. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. Analyst, M5, Flex 3, and SpectraMax L readers work differently. SpectraMax iD5. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. X. Reliable performance with a proven track record. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Versa Max. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 0 out of 5 $ 8,495. Uncontrolled Copy for Training and Reference Purposes Only . 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. Two high-energy xenon flash lamps: Reading capabilities. Figure 1. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. Microplate Readers. highthroughput alphascreen, alphascreen on plate reader Created Date:SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Published Dec 16, 2020 | Updated Dec 16, 2020. molecular interaction studies, and nucleic acid quantification, as well. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. #6590-0118) Filter 665/10 (Molecular Devices cat. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. 100 fmol 1536-well (8 µL) 0. Validated by Molecular Devices for the FLIPR reader. x. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Related products. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. Add 100 µl (96-well plate) or 25 µl (384-well plate) of cell suspension to each well of poly-D-lysine coated plates. Both readers were heated to 37ºC prior to setting up the assay. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . Shaking: Linear and Orbital. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 1 fmol luciferase per well. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 4 Equipment. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. This kit is designed for customers who. Alpha 384 HTS 96- and 384-well . 1. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. Bioz Stars score: 86/100, based on 9 PubMed citations. Backed by the. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. If the computer is connected to the Internet: Select Search Automatically for. 1 fmol luciferase per well. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. #S1205. Visit the supplier page or submit a quote to learn more. 314 low lamp level warning. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Temperature control: 4°C above ambient to 45°C. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 58% for the detection module and 2. An industry-renowned instrument for monitoring GPCRs and ion channels. These advertising cookies will no longer be set for this browser and device. Biotek Synergy 2 Plate Reader Multi-Mode. 4 Equipment. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. VIS absorbance detection. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. System setup and calibration are controlled from within SoftMax Pro Software. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. † Bottom mounting for SpectraMax Paradigm Readers only. The plate reader can measure multiple readouts, including UV and. Expand the Microplates tree. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. Step 1: Open the SoftMax Pro IMAP FP protocol. #S1207. A. #ID5) HTRF Detection System (Molecular Devices cat. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. Contact; Bioz Stars; Bioz vStars; 86 : Buy from Supplier : Structured Review. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Find our technical support information along with contact details. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Absorbance cuvette port. 5 pM 2. Typically, the full spectrum of signal from. 030 fmol Fluorescence Polarization (FP). The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. 96-, 384-, and 1536-well microplates. Four wash/rinse bottles are included and configurable for both 96- and 384. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 100 fmol luciferase per well. The SpectraMax ABS Plus reader utilizes Molecular . Note: These settings are based on necessary cookies. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 340 PC384. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Features. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. No accessories are needed and SoftMax® Pro Software. The FLIPR 384 system comes complete with 384. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Important things to consider when purchasing a multimode microplate reader. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. 96-384. Add 10 µL of ADP Detection Mix to those rows. 96-, 384-, and 1536. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 2. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. x. , San Diego, CA, USA) such that the. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The FLIPR 384 system comes complete with 384. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 5. easy to use and read multiple plates very quickly. For more sample throughput, the system can6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research.